Neitz, Albert Walter HermanVermeulen, N.M.J.Viljoen, G.J.Bigalke, R.D.2017-11-132017-11-1320171987Neitz, AWH, Vermeulen, NMJ & Viljoen, GJ 1987, 'Purification of Cowdria ruminantium by density gradient centrifugation', Onderstepoort Journal of Veterinary Research, vol. 54, no. 3, pp. 223-231.0330-2465http://hdl.handle.net/2263/63125The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.The isolation of Cowdria ruminantium by differential and isopycnic density gradient centrifugation is reviewed with special reference to the suitability of Percoll as density gradient medium. Infected sheep brain, Amblyomma hebraeum nymphae and various mouse organs were used as starting material. By these methods, partially purified viable populations of the organism with distinctly different densities were obtained. The conclusions are based upon results of analyses of density fractions by inoculation into sheep or mice, protein determination, electron microscopy and enzyme-linked immunosorbent assay. Morphological differences were observed in the density fractions obtained from infected brain tissue and A. hebraeum.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle