Akanmu, Abiodun MayowaVan Marle-Koster, EsteHassen, AbubekerAdejoro, Festus Adeyemi2024-05-142024-05-142023-12Akanmu, A.M., Van Marle-Koster, E., Hassen, A. et al. 2023, 'Datasets of shotgun metagenomics evaluation of rumen microbiota of South African mutton merino sheep', Data in Brief, vol. 51, art. 109629, pp. 1-5. https://DOI.org/10.1016/j.dib.2023.109629.2352-340910.1016/j.dib.2023.109629http://hdl.handle.net/2263/95967DATA AVAILABILITY : Methane mitigation using medicinal plant extracts (Original data) (NCBI Bioproject)The rumen microbial consortium plays a crucial role in the production performance and health of the ruminant animal. They are responsible for breaking down complex plant materials such as cellulose and hemicellulose to release usable energy by the host animal. Rumen microbial diversity manipulation through dietary strategies can be used to achieve several goals such as improved feed efficiency, reduced environmental impact or better utilization of low-quality forages. The dataset, deposited in the National Centre for Biotechnology Information SRA with project number PRJNA775821, comprises sequenced DNA extracted from the rumen content of 16 South African Merino sheep supplemented with different plant extracts. Illumina HiSeq TM 60 0 0 technology was utilised to generate a total of approximately 46.7 Gb in raw nucleotide data. The data consists of 700,318,582 sequences, each with an average length of 184 base pairs. Taxonomic an- notation conducted through the MG-RAST server showed the dominant phylum averages are Bacteroidetes (51 %) and Firmicutes (28 %), while Euryarchaeota, Actinobacteria, and Proteobacteria each account for approximately 3 % of the population. This dataset also enables us to identify and profile all expressed genes related to metabolic and chemical processes. The dataset is a valuable tool, offering insights that can lead to enhanced sustainability, profitability and reduced environmental impact within the context of ruminant pro- duction process.en© 2023 The Author(s). This is an open access article under the CCBY-NC-ND license.Shotgun approachRumen microbesPlant extractsFunctional genesIlluminaRumen metagenomeMG-RASTSDG-02: Zero hungerSDG-03: Good health and well-beingArticle