Mataseje, L.F.Pitout, Johann D.D.Croxen, M.Mulvey, M.R.Dingle, T.C.2024-06-072024-06-072023-10Mataseje, L.F., Pitout, J., Croxen, M. et al. 2023, 'Three separate acquisitions of blaNDM‑1 in three different bacterial species from a single patient', European Journal of Clinical Microbiology & Infectious Diseases, vol. 42, pp. 1275-1280. https://DOI.org/10.1007/s10096-023-04651-4.0934-9723 (print)1435-4373 (online)10.1007/s10096-023-04651-4http://hdl.handle.net/2263/96345DATA AVAILABILITY : Sequence data was uploaded to NCBI (BioProject PRJNA948358).To investigate the acquisition and relatedness of New Delhi Metallo-beta-lactamase among multiple separate species from one patient. Five isolates from three species (Pseudomonas aeruginosa; Pa, Acinetobacter baumannii; Ab and Proteus mirabilis; Pm) suspected of harbouring a carbapenemase were investigated by phenotype (antimicrobial susceptibilities) and whole genome sequencing. Epidemiological data was collected on this patient. Three different carbapenemase genes were detected; blaVIM-1 (Pa; ST773), blaOXA-23 (Ab, ST499) and blaNDM-1 identified in all isolates. NDM regions were found chromosomally integrated in all isolates. Data showed no evidence of NDM-1 transfer within this patient suggesting the enzyme was acquired in three separate events.en© The Author(s) 2023. This article is licensed under a Creative Commons Attribution 4.0 International License.CarbapenemasesCanadaNew Delhi metallo-beta-lactamasePseudomonasAcientobacterProteusSDG-03: Good health and well-beingArticle