Ambrosio, R.E.Visser, Elizabeth S.Koekhoven, YolandaKocan, Katherine M.Bigalke, R.D.2014-10-162014-10-1620141988Ambrosio, RE, Visser, ES, Koekhoven, Y & Kocan, KM 1988, 'Hybridization of DNA probes to A. marginale isolates from different sources and detection in Dermacentor andersoni ticks', Onderstepoort Journal of Veterinary Research, vol. 55, no. 4, pp. 227-229.0330-2465http://hdl.handle.net/2263/42387The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.DNA from the Washington, South-Idaho, Virginia and Florida isolates of Anaplasma marginale was hybridized to probes specific for Anaplasma centrale and A. marginale. The A. centrale probes AC-2 and AC-4 hybridized to identical bands on all of these isolates. The hybridization pattern suggests that the Virginia, Florida and the South African isolates are similar. A number of bands were obtained with the Washington isolate which differed from those obtained with the other isolates. Probe AC-2 could be developed to identify relatedness among Anaplasma isolates. Probe AC-2 detected A. marginale DNA in midgut material from infected Dermacentor andersoni ticks. No hybridization was obtained with DNA from salivary gland tissues from these infected ticks.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle