Viljoen, Gerrit JohannesVermeulen, N.M.J.Oberem, P.T.Bezuidenhout, J. DürrPutterill, John FraserVisser, L.Bigalke, R.D.2015-04-242015-04-2420141985Viljoen, G.J., Vermeulen, N.M.J., Oberem, P.T. et al. 1985, 'Isolation of Cowdria ruminantium by cellular affinity chromatography and detection by an enzyme-linked immunosorbent assay’, Onderstepoort Journal of Veterinary Research, vol. 52, no. 4, pp. 227-232.0330-2465http://hdl.handle.net/2263/44702The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.The isolation of Cowdria ruminantium by means of wheat germ lectin affinity chromatography as described in this paper permits the recovery of partially purified viable organisms under mild conditions in short time. These conclusions are based upon results of analyses of column fractions by intravenous inoculation into sheep, protein determination, electronmicroscopy and enzyme-linked immunosorbent assay. The entire purification procedure could be completed in 4-5 hours using only either infected sheep tissue or nymphae as starting material.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle