Patterton, H.G.Potgieter, D.J.J.Bigalke, R.D.2015-04-022015-04-0220141985Verschoor, J.A., Patterton, H.G. & Potgieter, D.J.J. 1985, 'Enrichment of a fraction toxic to guinea-pigs from Pachystigma pygmaeum (Schltr.) Robyns’, Onderstepoort Journal of Veterinary Research, vol. 52, no. 2, pp. 101-104.0330-2465http://hdl.handle.net/2263/44234The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Pachystigma pygmaeum is one of several species of rubiaceous plants which cause delayed heart failure among ruminants after their ingestion at relatively high doses. Using guinea-pigs for toxicity determinations, we were able to separate and enrich a toxic fraction from a fermentation extract of the plant material by countercurrent distribution. It contained virtually no potassium salts, passed through a 500 dalton selective membrane, exhibited lability under acid conditions and was toxic at 1 g/kg per os, with a delayed response of 3-4 days.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle