Josemans, A.I.Boomker, Jacob Diederik Frederik2011-12-022011-12-0220112003Zweygarth, E & Josemans, AI 2003, 'In vitro infection by Ehrlichia ruminantium of baby hamster kidney (BHK), Chinese hamster ovary (CHO-K1) and Madin Darby bovine kidney (MDBK) cells'. Onderstepoort Journal of Veterinary Research, vol. 70, no. 2, pp. 165-168.0030-2465http://hdl.handle.net/2263/17687The articles have been scanned with a HP Scanjet 8300; 600dpi, saved in TIFF format. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.The Welgevonden stock of Ehrlichia ruminantium, aetiological agent of heartwater, was propagated in baby hamster kidney (BHK) cells, Chinese hamster ovary (CHO-K1) cells and Madin Darby bovine kidney (MDBK) cells. The cultures required supplementation of the medium with cycloheximide for reliable growth of E. ruminantium. Growth of the Welgevonden stock in BHK and CHO-K1 cells could lead to the development of suspension cultures suitable for the mass production of E. ruminantium for an inactivated elementary body vaccine.en© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineBHK cellsCHO-K1 cellsCycloheximideEhrlichia (Cowdria) ruminantiumHeartwaterIn vitro cultivationMDBK cellsVeterinary medicine -- South AfricaHeartwater -- South AfricaTick-borne diseases in animalsArticle