Minnaar, P.P.McCrindle, Robert IanNaude, T.W.Botha, C.J. (Christoffel Jacobus)Verwoerd, Daniel Wynand2012-05-312012-05-3120122000Minnaar, PP, McCrindle, RI, Naude, TW & Botha, CJ 2000, 'Investigation of biological samples for monofluoroacetate and Dichapetalum cymosum poisoning in southern Africa’. Onderstepoort Journal of Veterinary Research, vol. 67, no. 1, pp. 27-30.0330-24657006558662O-5991-2014http://hdl.handle.net/2263/19026The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A need has existed for the accurate identification of monofluoroacetate (MFA) poisoning in southern Africa. The development of a new method for the determination of MFA has made the analysis of a variety of biological samples (n=50) feasible. The method has been used in the laboratory over 24 months. Monofluoroacetate was present in 66% of samples from cases of suspected poisoning, reflecting the extent of the problem. Stability of MFA in samples was also determined so as to have a time-bound baseline for the acceptance of samples submitted. It was found that there was a decrease in the level of MFA and, after 14 days at room temperature, only 50% of the spiked dose could be identified. It is suggested that samples be analyzed within 7 days of mortality if they not kept frozen.© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineDichapetalum cymosumGifblaarLiquid chromatographyMonofluoroacetate poisoningVeterinary medicine -- South AfricaVeterinary medicine -- ResearchVeterinary toxicology -- South AfricaPoisoning in animalsInvestigation of biological samples for monofluoroacetate and Dichapetalum cymosum poisoning in southern AfricaArticle