Titoy, G.A.P.Verwoerd, Daniel Wynand2013-08-152013-08-1520131996Titoy, GAP 1996, 'Cryopreservation of sheathed third-stage larvae of Nematodirus spathiger’. Onderstepoort Journal of Veterinary Research, vol. 63, no. 1, pp. 63.0330-2465http://hdl.handle.net/2263/22035The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A technique for the cryopreservation of sheathed third-stage larvae of Nematodirus spathiger is described. It consists of incubating larvae in 5% (v/v) ethylene glycol for 20 min at room temperature, followed by 1 min of incubation in 40% (v/v) ethylene glycol at 0°C. The average recovery of adult worms was 28,5% for two adult sheep infected with these larvae.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineCryopreservationSheathed third-stage larvaeNematodirus spathigerVeterinary medicine -- South AfricaArticle