Van Wyngaardt, WouterDu Plessis, D.H.Van Wyngaardt, SandraVerwoerd, Daniel Wynand2013-11-192013-11-1920131992Van Wyngaardt, W, Du Plessis, DH, Van Wyngaardt, S & Verschoor, JA 1992, 'Production and properties of monoclonal antibodies against African horsesickness virus serotype 3', Onderstepoort Journal of Veterinary Research, vol. 59, no. 2, pp. 129-133.0330-2465http://hdl.handle.net/2263/32469The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Four polyethylene glycol-mediated cell fusions yielded a total of 23 monoclonal antibodies (McAbs) specific for African horsesickness virus (AHSV). Two recognised the major core structural polypeptide, VP7, while one each was specific for the outer capsid proteins, VP2 and VP5. The remainder co-precipitated both VP2 and VP7. An inhibition ELISA and radio-immunoprecipitation revealed two types of co-precipitating McAbs, distinguishable from each other by the different relative amounts of the two proteins they precipitated. Only co-precipitating McAbs reduced the size and number of plaques formed by AHSV on VERO cell monolayers, but even at low dilution did not completely abolish virus infectivity. A McAb specific for VP7 showed potential as a group-reactive diagnostic reagent since guinea pig antisera to all nine serotypes of AHSV, as well as an anti-serotype 4 horse serum and an anti-serotype 3 rabbit serum, inhibited its binding in ELISA to AHSV serotype 3.en© South Africa. Dept. of Agricultural Technical Services. ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineAHSVVeterinary medicine -- South AfricaAfrican horse sickness -- South AfricaArticle