Josemans, A.I.Boomker, Jacob Diederik Frederik2012-03-062012-03-0620122001Zweygarth, E & Josemans, AI 2001, 'Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 2, pp. 155-157.0030-2465http://hdl.handle.net/2263/18399The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.The Welgevonden stock of Cowdria ruminantium, aetiologic agent of heartwater, was continuously propagated in DH82 cells, a continuous canine macrophage-monocyte cell line. Cultures of DH82 cells were readily infected provided that the culture medium was supplemented with cycloheximide. Cultures were split at regular 3-day intervals and infection rates ranged between 60% and 95%. Cultures were continuously propagated through more than 125 passages over a period of more than one year.© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineCanine macrophage-monocyte cell lineCowdria ruminantiumCycloheximideDH82HeartwaterIn vitro cultivationVeterinary medicine -- South AfricaTick-borne diseases in animalsHeartwater -- South AfricaCommunicable diseases in animalsContinuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell lineArticle