Hough, BiancaWingfield, Michael J.Read, David Alan2024-12-122024-12-122024-12Hough, B., Wingfield, B., Read, D. 2024, 'Identification and characterization of mycoviruses in transcriptomes from the fungal family ceratocystidaceae', Virus Genes, vol. 60, pp. 696-710. https://DOI.org/10.1007/s11262-024-02112-4.0920-8569 (print)1572-994X (online)10.1007/s11262-024-02112-4http://hdl.handle.net/2263/99920DATA AVAILABILITY : The transcriptomes used in the identification of viral sequences in this study are publicly available on the National Center for Biotechnology Information Sequencing Reads Archive. The accession numbers for these are indicated in Table 1, provided within the manuscript. The sequence data of all viruses identified this study have been deposited in the National Center for Biotechnology Information with the primary accession codes BK065014-BK065021.Mycoviruses pervade the fungal kingdom, yet their diversity within various fungal families and genera remains largely unexplored. In this study, 10 publicly available fungal transcriptomes from Ceratocystidaceae were analyzed for the presence of mycoviruses. Despite mycovirus associations being known in only four members of this family, our investigation unveiled the discovery of six novel mycoviruses. The majority of these mycoviruses are composed of positive sense single stranded RNA and are putatively assigned to the viral family Mitoviridae (with tentative classification into the genera Unuamitovirus and Duamitovirus). The double stranded RNA viruses, however, were associated with the family Totiviridae (with tentative classification into the genus Victorivirus). This study also revealed the discovery of an identical unuamitovirus in the fungal species Thielaviopsis ethacetica and Thielaviopsis paradoxa. This discovery was notable as these fungal isolates originated from distinct geographical locations, highlighting potential implications for the transmission of this mitovirus. Moreover, this investigation significantly expands the known host range for mycoviruses in this family, marking the initial identification of mycoviruses within Ceratocystis platani, Thielaviopsis paradoxa, Thielaviopsis ethacetica, and Huntiella omanensis. Future research should focus on determining the effects that these mycoviruses might have on their fungal hosts.en© The Author(s) 2024. Open access. This article is licensed under a Creative Commons Attribution 4.0 International License.MycovirusesCeratocystidaceaeMitovirusesThielaviopsisCeratocystisSDG-15: Life on landArticle