Mukorera, VaraidzoKirberger, Robert M.Mabeta, Peaceful LucyDvir, Eran2014-02-032014-02-032013-11Mukorera, V, Kirberger, RM, Mabeta, P & Dvir, E 2013, 'Vascular endothelial growth factor concentrations in dogs with Spirocercosis', Journal of Veterinary Internal Medicine, vol. 27, no. 6, pp. 1642-1645.0891-6640 (print)1939-1676 (online)10.1111/jvim.1217970045861827004445917N-8699-2014G-9725-201412791859400http://hdl.handle.net/2263/33205BACKGROUND : Vascular endothelial growth factor (VEGF) is a potent proangiogenic factor associated with tumor development.Spirocerca lupi is a nematode of canids that induces an esophageal nodule that progresses to a sarcoma in 25% of cases. Determination of neoplastic transformation is challenging and usually based on endoscopy-guided biopsies under general anesthesia, an expensive procedure that often yields nondiagnostic, necrotic samples. HYPOTHESIS : Circulatory VEGF concentrations are increased in dogs with neoplastic spirocercosis and can distinguish between dogs with neoplastic and nonneoplastic disease. ANIMALS : A total of 24 client-owned dogs, 9 nonneoplastic, 9 neoplastic, and 6 controls. METHODS : Case-control study. Plasma and serum VEGF concentrations at the time of diagnosis were compared with those of healthy controls. Measurement of VEGF was performed using a canine-specific ELISA. Kruskal-Wallis and Dunn’s tests were used for statistical analysis with significance set at P < .05. RESULTS : The median plasma VEGF concentrations of dogs with neoplastic spirocercosis were 629 pg/mL (range, 282– 2,366) higher than both the nonneoplastic (<39.5 pg/mL; range, <39.5–716) and control dogs (<39.5 pg/mL; all values, <39.5; P = .0003). The median serum VEGF concentration of the neoplastic dogs was 69 pg/mL (range, <39.5–212) higher than the nonneoplastic (<39.5 pg/mL; range, <39.5–44.13) and control dogs (<39.5 pg/mL; all values, <39.5; P = .001). CONCLUSIONS AND CLINICAL IMPORTANCE : Both plasma and serum VEGF concentrations can be used to differentiate nonneoplastic and neoplastic spirocercosis. The role of VEGF in neoplastic transformation of S. lupi-induced nodules and the potential utility of anti-VEGF drugs in spirocercosis-induced sarcoma warrant further investigation.en© 2013 by the American College of Veterinary Internal MedicineCanine esophagusNeoplasticNonneoplasticVascular endothelial growth factor (VEGF)Dogs -- DiseasesPostprint Article