Du Toit, P.J.Alexander, R.A.Neitz, W.O.Du Toit, P.J.Union of South Africa. Dept. of Agriculture2015-07-142015-07-1420151933Du Toit, PJ, Alexander, RA & Neitz, WO 1933, 'The immunization of mules with formalysed horsesickness virus. II', Onderstepoort Journal of Veterinary Science and Animal Industry, vol. 1, no. 1, pp. 21-24.http://hdl.handle.net/2263/48685The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.In a previous communication [this Bull. 3. 538.] it was shown that mules could be protected against horsesickness by a suitable formalinized vaccine. Further work has confirmed these results and indicated that a spleen virus attenuated by 1:2, 000 formalin protected against a 1:3, 500 concentration, while mules which survived the double inoculation were solidly immune. The method was not entirely devoid of danger as a mortality rate from the vaccine of about 5 per cent may be expected. The substitution of Tyrode's solution in place of saline as a diluent for the spleen emulsions failed to improve the vaccine.en©Union of South Africa, Dept. of Agriculture (original). ©University of Pretoria, Dept. of Library Services (digital).FormaldehydeVeterinary medicineVeterinary reportsSouth AfricaVeterinary medicine -- South AfricaAfrican horse sickness -- South AfricaOrbivirus infections in animalsVeterinary vaccinesArticle