South Africa. Dept. of Agricultural Technical ServicesTustin, R.C.De Lange, M.Reinecke, R.K.Walker, Jane B.De Kock, V.E.2015-07-082015-07-0820131970Oellermann, RA 1970, 'Plaque formation by African horsesickness virus and characterization of its RNA', Onderstepoort Journal of Veterinary Research, vol. 37, no.2 , pp. 137-144.http://hdl.handle.net/2263/46332The journals have been scanned in colour with a HP 5590 scanner; 600 dpi. Adobe Acrobat v.11 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A method of plaque assay for African horsesickness virus (AHSV) is described. Three cell lines were tested of which BHK21 was the most satisfactory. With an increase in Actinomycin D concentration, an increased inhibition of infective virus production was observed. Sucrose gradient sedimentation analysis of AHSV-RNA revealed at least six components. Eight components were resolved by polyacrylamide gel electrophoresis. The molecular weight of the components varied from 0.5 x 10⁶ to 2.8 x 10⁶ daltons, with a total molecular weight estimate of 1.5 x 10⁷ daltons for 10 segments in the viral genome. The significance of the relationship between AHSV, bluetongue virus and reovirus is discussed.en©South Africa. Dept. of Agricultural Technical Services (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary reportsSouth AfricaVeterinary medicine -- South AfricaAfrican horse sicknessHorses -- Virus diseasesOrbivirus infections in animalsArticle