Vorster, C.J.J. (Christiaan Jacob Johan)Joubert, Annie M.2011-05-122011-05-122010-06Vorster, C & Joubert, A 2010, 'In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line', Biocell, vol. 34, no. 2, pp. 71-79. [http://www.scielo.org.ar/scielo.php/script_sci_serial/lng_en/pid_0327-9545/nrm_iso]0327-9545 (print)1667-5746 (online)http://hdl.handle.net/2263/16569In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM’s in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4μM. In addition, mitotic indices revealed that 2ME-BM induces a G2M block. The latter was confirmed by flow cytometric analyses where increased sub-G1 and G2/M fractions, as well as an increase in cyclin B1 levels were observed. Further in vitro research into the mechanism of this potentially useful anticancer compound is thus warranted.enCentro Regional de Investigaciones Cientificas Y Tecnologicas / Universidad Nacional de CuyoCancer2-methoxyestradiolSulphamoylated estradiol derivativeApoptosisAutophagyCancer -- Treatment -- ResearchArticle