Van Vuuren, A.S.Potgieter, F.T.Bigalke, R.D.Cameron, Colin McKenzieVerster, Anna J.M.Walker, Jane B.De Kock, V.E.2016-07-072016-07-0720161974Potgieter, FT & Van Vuuren, AS 1974, 'The transmission of Babesia bovis using frozen infective material obtained from Boophilus microplus larvae’, Onderstepoort Journal of Veterinary Research, vol. 41, no. 2, pp. 79-80.0330-2465http://hdl.handle.net/2263/54029The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Boophilus microplus larvae infected with Babesia bovis were removed from a susceptible ox approximately 72 hours after attachment, triturated and stored in liquid nitrogen refrigerator with dimethyl sulphoxide as cryoproctectant. When inoculated into splenectomised cattle the suspension was infective in the 2 animals into which it was injected intravenously but not in the 3rd, which was only injected subcutaneously. The prepatent period in the infected cattle was 10 and 11 days respectively.en©1974 ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original) ©2016 University of Pretoria. Department of Library Services (digital)Veterinary medicineVeterinary medicine -- South AfricaArticle