Zablocki, OlivierPietersen, Gerhard2014-05-292014-05-292014-08Zablocki, O & Pietersen, G 2014, 'Characterization of a novel citrus tristeza virus genotype within three cross-protecting source GFMS12 sub-isolates in South Africa by means of Illumina sequencing', Archives of Virology, vol. 159, no. 8, pp. 2133-2139.0304-8608 (print)1432-8798 (online)10.1007/s00705-014-2041-3http://hdl.handle.net/2263/39914Tristeza disease (caused by citrus tristeza virus, CTV) is currently controlled in South Africa by means of cross-protection. In this study, we characterized the CTV populations of three grapefruit mild strain 12 (GFMS12) single-aphid-transmission-derived sub-isolates at the whole-genome level using Illumina sequencing technol­ogy. A novel South African isolate (CT-ZA3, of the T68 genotype) was shown to be the dominant genotype in all GFMS12 sub-isolates tested, along with reads unique to various other genotypes occurring as minor components. Uncertainty remains as to the significance of these minor components.en© Springer-Verlag Wien 2014. The original publication is available at : http://link.springer.com/journal/705.Triteza virusNext-generation sequencingGrapefruit mild strain 12 (GFMS12)Postprint Article