Barcelona, VeronicaHuang, YunfengCaceres, Billy A.Newhall, Kevin P.Hui, QinCerdena, Jessica P.Crusto, Cindy A.Sun, Yan V.Taylor, Jacquelyn Y.2022-12-142022-12-142022-08-11Barcelona, V.; Huang, Y.; Caceres, B.A.; Newhall, K.P.; Hui, Q.; Cerdeña, J.P.; Crusto, C.A.; Sun, Y.V.; Taylor, J.Y. Experiences of Trauma and DNA Methylation Profiles among African American Mothers and Children. International Journal of Molecular Sciences. 2022, 23, 8951. https://doi.org/10.3390/ijms23168951.1661-6596 (print)1422-0067(online)10.3390/ijms23168951https://repository.up.ac.za/handle/2263/88776Potentially traumatic experiences have been associated with chronic diseases. Epigenetic mechanisms, including DNA methylation (DNAm), have been proposed as an explanation for this association. We examined the association of experiences of trauma with epigenome-wide DNAm among African American mothers (n = 236) and their children aged 3–5 years (n = 232; N = 500), using the Life Events Checklist-5 (LEC) and Traumatic Events Screening Inventory—Parent Report Revised (TESI-PRR). We identified no DNAm sites significantly associated with potentially traumatic experience scores in mothers. One CpG site on the ENOX1 gene was methylome-wide-significant in children (FDR-corrected q-value = 0.05) from the TESI-PRR. This protein-coding gene is associated with mental illness, including unipolar depression, bipolar, and schizophrenia. Future research should further examine the associations between childhood trauma, DNAm, and health outcomes among this understudied and high-risk group. Findings from such longitudinal research may inform clinical and translational approaches to prevent adverse health outcomes associated with epigenetic changes.en© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).DNA methylationTraumaAdverse childhood experiences (ACES)EpigenomicsWomenAfrican AmericansDeoxyribonucleic acid (DNA)Article