Lee, ImchangPodolich, OlgaBrenig, BertramTiwari, SandeepAzevedo, VascoDe Carvalho, Daniel SantanaTrovatti Uetanabaro, Ana PaulaGoes-Neto, AristotelesAlzahrani, Khalid J.Reva, Oleg N.Kozyrovska, NataliaDe Vera, Jean-PierreBarh, DebmalyaKim, Bong-Soo2023-07-262023-07-262022-08Lee, I., Podolich, O., Brenig, B. et al. 2022, 'Metagenome-assembled genomes of Komagataeibacter from Kombucha exposed to Mars-like conditions reveal the secrets in tolerating extraterrestrial stresses', Journal of Microbiology and Biotechnology, vol. 32, no. 8, pp. 967-975, doi : 10.4014/jmb.2204.04009.1017-7825 (print)1738-8872 (online)10.4014/jmb.2204.04009http://hdl.handle.net/2263/91636Kombucha mutualistic community (KMC) is composed by acetic acid bacteria and yeasts, producing fermented tea with health benefits. As part of the BIOlogy and Mars EXperiment (BIOMEX) project, the effect of Mars-like conditions on the KMC was analyzed. Here, we analyzed metagenome-assembled genomes (MAGs) of the Komagataeibacter, which is a predominant genus in KMC, to understand their roles in the KMC after exposure to Mars-like conditions (outside the International Space Station) based on functional genetic elements. We constructed three MAGs: K. hansenii, K. rhaeticus, and K. oboediens. Our results showed that (i) K. oboediens MAG functionally more complex than K. hansenii, (ii) K. hansenii is a keystone in KMCs with specific functional features to tolerate extreme stress, and (iii) genes related to the PPDK, betaine biosynthesis, polyamines biosynthesis, sulfate-sulfur assimilation pathway as well as type II toxin-antitoxin (TA) system, quorum sensing (QS) system, and cellulose production could play important roles in the resilience of KMC after exposure to Mars-like stress. Our findings show the potential mechanisms through which Komagataeibacter tolerates the extraterrestrial stress and will help to understand minimal microbial composition of KMC for space travelers.en© 2022 by the authors. Licensee KMB. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.Kombucha mutualistic community (KMC)KomagataeibacterMetagenome-assembled genome (MAG)Mars-like conditionWhole metagenomeArticle