Theodoridis, A.Nevill, E.M.Els, H.J.Boshoff, S.T.Bigalke, R.D.Morren, A.J.Verster, Anna J.M.Verwoerd, Daniel WynandWalker, Jane B.Cameron, Colin McKenzieGilchrist, Frances M.C.Steyn, P.J.J.2016-07-062016-07-0620161979Theodoridis, A, Nevill, EM, Els, HJ & Boshoff, ST 1979, 'Viruses isolated from Culicoides midges in South Africa during unsuccessful attempts to isolate bovine ephemeral fever virus’, Onderstepoort Journal of Veterinary Research, vol. 46, no. 4, pp. 191-198.0330-2465http://hdl.handle.net/2263/53830This article has been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-Format.Five viruses, unrelated to bovine ephemeral fever virus (BEFV), were isolated from Culicoides biting-midges collected during the summer months of the years 1968-69 and 1969-70 near a cattle herd in which cases of BEF occurred and at an open horse stable at Onderstepoort. These viruses were investigated by means of serological, electron-microscopical and physicochemical tests. It was established that 2 isolates, Cul. 1/69 and Cul. 2/69, were related to each other and belonged to the Palyam subgroup of the genus Orbivirus, that isolate Cul. 3/69 belonged to the equine encephalosis subgroup of the genus Orbivirus, while Cul. 1/70 was related to Akabane virus, which belongs to the Simbu subgroup of the family Bunyaviridae. One isolate, Cul. 5/69, though prevalent in the cattle population, could not be identified at this point. A brief serological survey indicated that the cattle in the nearby herd possessed antibodies against all the isolates except Cul. 3/69. BEFV could not be isolated in mice or in cultured cells from the wild-caught Culicoides.en©1979 ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©2016 University of Pretoria Department of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle