Eicker, A.2021-11-022021-11-0220211990*http://hdl.handle.net/2263/82492Dissertation (MSc)--University of Pretoria, 1990.Suitable agar or liquid media can be prepared from wheat straw extract to grow and maintain Pleurotus (HK 35) in the absence ofMEA or PDA agar medium and YPG liquid medium. Wheat straw spawn and sorghum grain spawn dehydrated at 40°C for 7h can be used to inoculate substrates treated with hot water in cultivation of oyster mushrooms in rural areas. The inoculated substrate can be cultured in surface or underground thatched structures constructed from locally available materials. In forested areas, the substrate can even be incubated amongst trees during cool humid seasons. The best results during this investigation were obtained using dehydrated sorghum spawn in hot water treated grass straw substrate cultivated in a surface grass hut structure.© 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.UCTDRural oyster mishroomDissertation