Wang, Zheng-WeiVreede, F.T.Mitchell, J.O.Viljoen, G.J.Boomker, Jacob Diederik Frederik2012-12-122012-12-1220122001Wang, Z, Vreede, FT, Mitchell, JO & Viljoen, GJ 2001, 'Rapid detection and differentiation of Newcastle disease virus isolates by a triple one-step RT-PCR’. Onderstepoort Journal of Veterinary Research, vol. 68, no. 2, pp. 131-134.0030-2465http://hdl.handle.net/2263/20686The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A triple one-step RT-PCR was developed to screen and differentiate virulent from avirulent Newcastle disease virus (NDV) isolates. Three sets of oligonucleotides were designed, each specific for amplifying NDV fusion protein gene-specific RNA from virulent, avirulent or all isolates respectively. The sensitivity of one-step RT-PCR was determined using viral RNA extracted from serially diluted NDV-infected allantoic fluid and found to be 10ˉ⁵ HA units. Application of one-step RT-PCR to various NDV samples, including wild-type virulent isolates and avirulent vaccine strains, demonstrated the potential for rapid identification (3-4 h) of NDV isolates as well as the differentiation of virulent from avirulent strains.© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineDetectionIdentificationNewcastle disease virus (NDV)RT-PCRTriton X-100 differentiation of NDV isolatesVeterinary medicine -- South AfricaArticle