Potgieter, F.T.Van Rensburg, L.Bigalke, R.D.Cameron, Colin McKenzieGilchrist, Frances M.C.Jordaan, E.Morren, A.J.Verster, Anna J.M.Verwoerd, Daniel WynandWalker, Jane B.Steyn, P.J.J.2015-12-072015-12-0720151982Potgieter, FT & Van Rensburg, L 1982, 'The effect of incubation and prefeeding of infected Rhipicephalus simus nymphae and adults on the transmission of Anaplasma marginale’, Onderstepoort Journal of Veterinary Research, vol. 49, no. 2, pp. 99-101.0330-2465http://hdl.handle.net/2263/51088The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Two batches of unfed Rhipicephalus simus nymphae carrying Anaplasma marginale were incubated for 72h and 96h respectively at 37°C. Fifty ticks were triturated at a time and the homogenates were used to infect susceptible cattle. Others were prefed on a bovine host for 72h before tick suspensions were prepared. The same procedure was followed, using a single batch of infected adult ticks. Incubation had no effect on the infectivity of the nymphal homogenates, but prefeeding of nymphae reduced the prepatent period by 8 days in l of the 2 attempts. In the case of the adult ticks both incubation and prefeeding reduced prepatent periods by 5 and 8 days respectively.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle