Alamnie, GetachewBekele, TigabuGirma, AbayenehMalgas, Samkelo2026-04-012026-04-012026-04Alamnie, G., Bekele, T., Girma, A. & Malgas, S. 2026, 'Prospecting, production and thermodynamic profiling of feather degrading keratinolytic protease from Bacillus subtilis GH2', Biocatalysis and Agricultural Biotechnology, vol. 73, art. 103979, pp. 1-12, doi : 10.1016/j.bcab.2026.103979.1878-818110.1016/j.bcab.2026.103979http://hdl.handle.net/2263/109382DATA AVAILABILITY : Data will be made available on request.Please read abstract in the article. HIGHLIGHTS • Dialysis and ammonium sulfate purification gave a purification yield of 1.99%. • Kinetic properties showed that the keratinase can bind to its substrate. • Thermodynamic parameters suggest that the enzyme is relatively thermostable.en© 2026 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).Bacillus subtilisKeratinaseKineticsPurificationThermal-denaturationArticle