Roux, JolandaWingfield, Michael J.Fourie, AristaNoeth, K.Barnes, Irene2021-11-112021-11-112020J Roux, MJ Wingfield, A Fourie, K Noeth & I Barnes (2020) Ceratocystis wilt on Eucalyptus: first record from South Africa, Southern Forests: a Journal of Forest Science, 82:1, 24-31, DOI: 10.2989/20702620.2019.1686687.2070-2620 (print)2070-2639 (online)10.2989/20702620.2019.1686687http://hdl.handle.net/2263/82639Wilt and death of an E. grandis × E. urophylla variety was recently observed in the Zululand region of KwaZulu-Natal, South Africa. Symptoms on the dying trees included a streaking pattern of discolouration in the sapwood and cambium of stems and roots. A fungus resembling a Ceratocystis sp. was consistently found sporulating on diseased material and isolated from the symptomatic root and stem tissue. DNA sequence analyses of the Ceratocystis isolates, including multiple gene regions, identified the isolates as C. eucalypticola. Multiple ITS types were identified among the isolates sequenced, similar to those found in other, closely related, Ceratocystis species. Artificial inoculations under field conditions confirmed the pathogenicity of the isolates. This is the first report of a Ceratocystis sp. causing a wilt disease of Eucalyptus in South Africa.en© 2020 NISC (Pty) Ltd. This is an electronic version of an article published in Southern Forests, vol. 82, no. 1, pp. 24-31, 2020. doi : 10.2989/20702620.2019.1686687. Southern Forests is available online at : http://www.tandfonline.comloi/tsfs20.CankerCeratocystidaceaeEmerging diseaseRoot infectionVascular wiltPostprint Article