Annandale, C.H. (Cornelius Henry)Schulman, M.L. (Martin)Kirkpatrick, R.D.2009-01-222009-01-222008-03Annandale, CH, Schulman, ML, & Kirkpatrick, RD 2008, 'The use of electrochemically activated saline as a uterine instillation in pony mares', Journal of the South African Veterinary Association, vol. 79, no. 1, pp. 36-38. [http://www.journals.co.za/ej/ejour_savet.html]0038-2809http://hdl.handle.net/2263/8667Twelve pony mares were randomly assigned to either a control or a treatment group and inseminated with fresh, raw semen from a single stallion of known fertility in a cross-over trial design. Pregnancy was diagnosed by transrectal ultrasound 12-14 days post-ovulation and then terminated by administration of a luteolytic dose of cloprostenol. Treatment mares received a uterine instillation of 100 m of electrochemically activated (ECA) saline 4-12 hours post-insemination. Control mares received no treatment post-insemination. Per cycle pregnancy rate was 58.3 % in the control group and 50 % in the treatment group. There was no statistical difference (P = 1.000) in pregnancy rate between the 2 groups. The principles of ECA and applications of ECA saline are discussed.enSouth African Veterinary AssociationElectrochemicalEndometritisFresh semenMaresSalinePoniesSemenReproductionFertilityArticle