Ehlers, Marthie MagdaleenCloete, Thomas Eugene2007-08-312007-08-311999-07Ehlers, MM & Cloete, TE 1999, Protein profiles of phosphorus- and nitrate-removing activated sludge systems, Water SA, vol. 25, no. 3, pp. 351-356. [http://www.wrc.org.za/publications_watersa.htm]1816-7950http://hdl.handle.net/2263/3408Samples from laboratory-scale activated sludge systems operating under spesific conditions which differ in phosphate-removing capabilities were obtained from the University of Cape Town. The total protein content of samples of these systems was used and the protein separated with SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Analysis of the total proteins was employed as a fingerprint to type and compare the diversity of the bacterial communities of P-and N-removing systems. Samples of six activated slugde systems (three N-and P-removing and three N-removing) were used in this study. Protein profiles indicated a high (>70%) correlation for all the systems. No difference was observed in the protein profiles of the bacterial communities of N-and P-removing or N-removing systems. We can therefore conclude that the same bacterial communities were present in P-and N-removing systems.328856 bytesapplication/pdfenWater Research CouncilSludge systemsProtein profilesPhosphorus- and nitrate-removingExtraction (Chemistry)Bacterial growthArticle