Machaba, Tambudzani C.Mahlo, Salome M.Eloff, Jacobus NicolaasNxumalo, WinstonMcGaw, Lyndy Joy2024-10-302024-10-302024-02Machaba, T.C., Mahlo, S., Eloff, J., Nxumalo, W. & McGaw, L., 2024, ‘Cytotoxicity, antifungal and antioxidant properties of Lonchocarpus capassa leaf extracts’, Journal of Medicinal Plants for Economic Development 8(1), a221. https://doi.org/10.4102/jomped.v8i1.221.2519-559X (print)2616-4809 (online)10.4102/jomped.v8i1.221http://hdl.handle.net/2263/98847Sections of this manuscript (12%) are published in a thesis submitted in partial fulfilment of the requirements for the degree of PhD and Masters in the University of Limpopo and University of Pretoria entitled ‘isolation, characterisation and cytotoxicity of antifungal compounds present in medicinal plants used against cryptococcus neoformans in vhembe district, limpopo province’ and ‘Ethnobotanical survey of medicinal plants with antifungal activities in Makhado Local Municipality, Limpopo Province, South Africa’ respectively with Supervisor: Prof. S. Mahlo, available here: http:// ulspace.ul.ac.za/bitstream/handle/10386/4262/makhado_ tc_2018.pdf?isAllowed= y&sequence=1 and http://ulspace. ul.ac.za/bitstream/handle/10386/4291/machaba_tc_2023. pdf?sequence=1&isAllowed=y.DATA AVAILABILITY : The data used to support the findings of this study is available from the corresponding author, S.M., upon reasonable request.BACKGROUND : Lonchocarpus capassa is a medicinal plant used to treat diseases such as fungal infections, diarrhoea, oral candidiasis, and stomach complaints in South Africa. AIM : The study aimed to investigate the cytotoxicity and antifungal compounds isolated from L. capassa leaf extracts. SETTING : The study was conducted in Muduluni village, Makhado Local Municipality, Limpopo province. METHODS : Leaf extracts were screened for antifungal activity against fungal pathogens: Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Bioassay-guided fractionation using column chromatography of the acetone extract led to the isolation of six antifungal compounds. Nuclear Magnetic Resonance spectroscopy and Mass Spectrometry were used for the identification of compounds. The antioxidant activity of the plant extracts was investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Cytotoxicity of isolated compounds was determined using the 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay against Vero monkey kidney cells. RESULTS : The plant extract had an excellent minimum inhibitory concentrations (MIC) value of 40 μg/mL against the microorganisms. Compound 1 was identified as Lupeol, Compound 3 as Friedelin, and Compound 4 as 6-(γ,γ-Dimethylallyl)-3’,4’-dimethoxy-6”,6”-dimethylpyrano-[2”,3”:7,8]-flavanone (Compound 4). Compounds 2 and 5 were not identified because of the presence of mixtures of long-chain fatty acids. Friedelin was the most active radical scavenger in the DPPH assay. The compounds were not toxic with an LC50 value of ˃ 0.2 mg/mL. CONCLUSION : Screening of medicinal plants could provide lead to the discovery of novel antifungal agents. CONTRIBUTION : The results support the traditional use of L. capassa to combat fungal infections in humans.en© 2024. The Author(s). Licensee: AOSIS. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.Lonchocarpus capassaAntifungal activityCytotoxicityMinimum inhibitory concentration (MIC)Fungal infectionsSDG-03: Good health and well-beingArticle