Senamela, TshiphiriKock, Marleen M.Becker, Piet J.Potgieter, Joachim2019-04-172019-04-172018-01-31Senamela T, Kock M, Becker P, et al. Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay. African Journal of Laboratory Medicine 2018;7(1), a658. https://DOI.org/10.4102/ajlm.v7i1.658.2225-2002 (print)2225-2010 (online)10.4102/ajlm.v7i1.658http://hdl.handle.net/2263/68993The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.en© 2018. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.Diagnostic laboratoriesSequencingPolymerase chain reaction (PCR)DetectionJanus kinase 2 V617F mutationHealth sciences articles SDG-03SDG-03: Good health and well-beingHealth sciences articles SDG-09SDG-09: Industry, innovation and infrastructureHealth sciences articles SDG-17SDG-17: Partnerships for the goalsArticle