Engelbrecht, SusanDe Villiers, TaniaSampson, Candice C.Zur Megede, JanBarnett, Susan W.Janse van Rensburg, Estrelita2007-07-312007-07-312001-11Engelbrecht, S, De Villiers, T, Sampson, CC, Zur Megede, J, Barnett, SW & Janse van Rensburg, E 2001,'Genetic analysis of the complete gag and env genes of HIV-1 subtype C primary isolates from South Africa', Aids Research and Human Retroviruses, vol. 17, no. 16, pp. 1533-1547. [http://www.liebertonline.com/aid?]0889-222910.1089/08892220152644250http://hdl.handle.net/2263/3217The publisher does not allow open access to the full text of this sequence note. If you however need access to the full text, please contact the Collection Administrator, or access the item via the e-journal collection.South Africa has one of the fastest growing HIV-1 epidemics, with an estimated 4.7 million people infected. To better understand the genetic diversity of this epidemic and its potential impact on vaccine development, we have cloned and sequenced the complete gag and env genes of 13 primary virus isolates. Phylogenetic analysis of our sequences and 69 complete env genes from the Los Alamos and GenBank databases revealed multiple subclusters within subtype C. The V3 loop region was relatively conserved in all our strains when compared with other subtypes, but the region immediately downstream was highly variable. No intersubtype recombinant forms were observed when comparing the gag and env sequences. Characterization of the complete gag and env genes enabled us to select specific strains for further vaccine development.579141 bytesapplication/pdfenMary Ann Liebert.HIV-1HIV (Viruses) -- Research -- South AfricaAIDS vaccines -- South AfricaHIV (Viruses) -- Genetic aspectsDisease susceptibility -- Genetic aspectsText