Strydom, Kathy-AnneIsmail, FarzanaMatabane, M.M.Z.Onwuegbuna, O.Omar, Shaheed VallyIsmail, Nabila2015-12-042015-09Strydom, KA, Ismail, F, Matabane, MMZ, Onwuegbuna, O, Omar, SV & Ismail, N 2015, 'Comparison of three commercial molecular assays for detection of rifampin and isoniazid resistance among Mycobacterium tuberculosis isolates in a high-HIV-prevalence setting', Journal of Clinical Microbiology, vol. 53, no. 9, pp. 3032-3034.0095-1137 (print)1098-660X (online)10.1128/JCM.01691-15http://hdl.handle.net/2263/51054In a head to head comparison of the MTBDRplus Version 2.0 (Hain Lifescience), Xpert® MTB/RIF (Cepheid) and the Anyplex™ MTB/NTM (Seegene) assays we demonstrate equal sensitivity (59/61; 96.7%) and specificity (53/54; 98.1%) for detecting rifampicin resistance with further analysis of discordances. The Xpert does not detect Isoniazid resistance while Anyplex showed high false positivity.en© 2015. American Society for Microbiology. All Rights Reserved.RifampicinIsoniazidMycobacterium tuberculosisHigh HIV prevalence settingPostprint Article