Du Plessis, D.H.Verwoerd, Daniel Wynand2014-08-212014-08-2120131992Du Plessis, DH 1992, 'Serological differentiation of five bluetongue virus serotypes in indirect ELISA'. Onderstepoort Journal of Veterinary Research, vol. 59, no. 2, pp. 119-122.0330-2465http://hdl.handle.net/2263/41461The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific for virus particles was able to distinguish heterologous serotypes from each other, but a sheep serum from an infected animal was not. An antiserum directed against VP2, the protein responsible for serotype specificity in neutralization tests, was not serotype-specific in ELISA and cross-reacted with other serotypes. The discriminatory ability of a BTV-4 antiserum was improved by cross-absorption with heterologous antigens. This greatly reduced the ELISA signals with heterologous serotypes and produced an antiserum that was effectively serotype-specific.en©South Africa. Dept. of Agricultural Technical Services. ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineBTVVeterinary medicine -- South AfricaArticle