Skorupskaite, KarolinaGeorge, Jyothis T.Veldhuis, Johannes D.Millar, Robert P.Anderson, Richard A.2018-09-122018-09-122018-04-05Skorupskaite, K., George, J.T., Veldhuis, J.D. et al. 2018, 'Neurokinin 3 receptor antagonism reveals roles for neurokinin B in the regulation of gonadotropin secretion and hot flashes in postmenopausal women', Neuroendocrinology, vol. 106, pp. 148-157.0028-3835 (print)1423-0194 (online)10.1159/000473893http://hdl.handle.net/2263/66540OBJECTIVES : Neurokinin B (NKB) and kisspeptin are obligate for normal gonadotropin secretion, and links between gonadotropin- releasing hormone (GnRH) pulsatility and vasomotor symptoms have been proposed. Using a selective NKB receptor (NK3R) antagonist, the role of NKB in the hypergonadotropic state in menopausal women was explored. METHODS : Eleven postmenopausal women were administered the NK3R antagonist MLE4901 at 40 mg twice daily orally for 7 days. Ten-minute blood sampling for 8 h was performed before and on the last day of NK3R antagonist treatment for luteinising hormone (LH) pulsatility analysis with kisspeptin-10 (0.3 μg/kg i.v. bolus) administered at 6 h on both days. Hot flash frequency and severity were self-reported for 7 days before and during NK3R antagonist administration. RESULTS : LH fell from 29.3 ± 4.1 to 24.4 ± 3.8 IU/L (p < 0.05) after 7 days of NK3R antagonist treatment, with no change in follicle-stimulating hormone (FSH). Basal (non-en© 2017 The Author(s) Published by S. Karger AG, Basel. This article is licensed under the Creative Commons Attribution 4.0 International License (CC BY) (http://www.karger.com/Services/ OpenAccessLicense).KisspeptinMenopauseHot flashesNeurokinin B (NKB)NKB receptor (NK3R)Gonadotropin-releasing hormone (GnRH)NuronsLuteinising hormone (LH)Gene expressionMenstrual cycleBody temperature (Tb)HypothalamusArcuate nucleusHypogonadotropic hypogonadismHormone secretionArticle