Van Wyk, Jan AucampDe Lange, M.Bigalke, R.D.Howell, P.G.Reinecke, R.K.Walker, Jane B.De Kock, V.E.2016-07-062016-07-0620161973Van Wyk, JA 1973, 'Studies on schistosomiasis. 4. Differential staining of live and dead cercariae after immobilization with physostigmin’, Onderstepoort Journal of Veterinary Research, vol. 40, no. 1, pp. 23-30.0330-2465http://hdl.handle.net/2263/53674The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A modified eosin technique similar to that used for differential staining of live and dead spermatozoa stained dead cercariae of Schistosoma mattheei red while those that were alive remained colourless and transparent. Prior to exposure to the dye live cercariae were immobilized with physostigmin, which was better for that purpose than five other substances tested. The lowest concentration of physostigmin to immobilize cercariae quickly and effectively was found to be 3,3 x 10-⁶ M.en© 1973 ARC - Onderstepoort and Faculty of Veterinary Science. University of Pretoria (original). © 2016 University of Pretoria. Department of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle