Fourie, NielErasmus, G.L.Schultz, R.A. (Rowena Anitra)Verwoerd, Daniel Wynand2013-09-122013-09-1220131995Fourie, N, Erasmus, GL, Schultz, RA & Prozesky, L 1995, 'Isolation of the toxin responsible for gousiekte, a plant-induced cardiomyopathy of ruminants in southern Africa’. Onderstepoort Journal of Veterinary Research, vol. 62, no. 2, pp. 77-87.0330-2465http://hdl.handle.net/2263/31699The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.Gousiekte was induced in sheep and goats by the oral and intravenous (i/v) administration of methanolic and ethanolic precipitates of aqueous extracts of Pavetta harborii. Further purification of the toxic principle was carried out by dialysis and ion-exchange chromatography. The toxin was eluted from the cation-exchange resin with 1 mol/l of ammonium acetate and finally purified on Sephadex G-10 and silica gel. Spots on thin-layer chromatographic plates (TLC) were visualized with ninhydrin. Gousiekte was induced with the pure toxin after i/v administration in goats. The yield of pure toxin was about 10 mg/kg of dried leaves. A compound, indistinguishable from the above on TLC, was also isolated from the gousiekte-inducing plants Pavetta schumanniana, Fadogia homblei and Pachystigma pygmaeum.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineGousiekteCardiomyopathyCardiac failurePlant poisoningCardiotoxinVeterinary medicine -- South AfricaArticle