Rothmann-Meyer, WiekolizeNaidoo, KershneyDe Waal, Pamela Jean2024-09-102024-09-102024-09Rothmann-Meyer, W., Naidoo, K. & De Waal, P.J. 2024, 'Spirocerca lupi draft genome, vaccine and anthelmintic targets', Molecular and Biochemical Parasitology, vol. 259, art. 111632, pp. 1-9, doi : 10.1016/j.molbiopara.2024.111632.0166-6851 (print)1872-9428 (online)10.1016/j.molbiopara.2024.111632http://hdl.handle.net/2263/98097SUPPLEMENTARY MATERIAL 1 : S1 Known anthelmintic targets. SUPPLEMENTARY MATERIAL 2 : S2 New anthelmintic targets: Bottom up search. SUPPLEMENTARY MATERIAL 3 : S3 Known vaccine and immune modulators. SUPPLEMENTARY MATERIAL 4 : S4 New Vaccine targets. SUPPLEMENTARY MATERIAL 5 : S5 Singletons KEGG results. SUPPLEMENTARY MATERIAL 6 : S6 Singletons InterPro Scan results. SUPPLEMENTARY MATERIAL 7 : S7 Canid Parasites and C elegans Orthovenn2. SUPPLEMENTARY MATERIAL 8 : S8 Canid Parasites only Orthovenn2.Spirocerca lupi is a parasitic nematode affecting predominantly domestic dogs. It causes spirocercosis, a disease that is often fatal. The assembled draft genome of S. lupi consists of 13,627 predicted protein-coding genes and is approximately 150 Mb in length. Several known anthelmintic gene targets such as for β-Tubulin, glutamate, and GABA receptors as well as known vaccine gene targets such as cysteine protease inhibitor and cytokines were identified in S. lupi by comparing orthologs of C. elegans anthelmintic gene targets as well as orthologs to known vaccine candidates. New anthelmintic targets were predicted through an inclusion-exclusion strategy and new vaccine targets were predicted through an immunoinformatics approach. New anthelminthic targets include DNA-directed RNA polymerases, chitin synthase, polymerases, and other enzymes. New vaccine targets include cuticle collagens. These gene targets provide a starting platform for new drug identification and vaccine design.en© 2024 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC license.Spirocerca lupi genomeSpirocerca lupi anthelminticSpirocerca lupi vaccineGastrointestinal parasiteInclusion-exclusionImmunoinformaticSDG-03: Good health and well-beingArticle