Brayton, Kelly A.Bothma, G.C.Vogel, S.W.Allsopp, B.A.Verwoerd, Daniel Wynand2017-06-292017-06-2920121997Brayton, KA, Bothma, GC, Vogel, SW & Allsopp, BA 1997, 'Development of the OPgun™ for bombardment of animal tissues’, Onderstepoort Journal of Veterinary Research, vol. 64, no. 2, pp. 153-156.0330-2465http://hdl.handle.net/2263/61215The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A simple and inexpensive particle-bombardment device, the OPgun™, was constructed for the delivery of DNA into animal tissues. This device is based on the particle-inflow gun first described for plant-cell transfection. The delivery of tungsten particles into the epidermis of the mouse ear, without the use of vacuum and without causing damage to the tissue, was demonstrated. The system was also shown to be capable of inducing antibodies to a foreign gene in mice.en© ARC-Onderstepoort (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle