Wolmarans, ElizeMqoco, T.V. (Thandi Vuyelwa)Stander, AndreNkandeu, SandraSippel, Katherine H.McKenna, RobertJoubert, Annie M.2015-03-092015-03-092014-03Wolmarans, E, Mqoco, TV, Stander, A, Nkandeu, SD, Sippel, KH, Mckenna, R & Joubert, AM 2014, 'Novel estradiol analogue induces apoptosis and autophagy in esophageal carcinoma cells', Cellular & Molecular Biology Letters, vol. 19, no. 1, pp. 98-115.1425-8153 (print)1689-1392 (online)10.2478/s11658-014-0183-7http://hdl.handle.net/2263/43895Cancer is the second leading cause of death in South Africa. The critical role that microtubules play in cell division makes them an ideal target for the development of chemotherapeutic drugs that prevent the hyperproliferation of cancer cells. The new in silico-designed estradiol analogue 2-ethyl-3-O-sulfamoyl-estra-1,3,5(10)16-tetraene (ESE-16) was investigated in terms of its in vitro antiproliferative effects on the esophageal carcinoma SNO cell line at a concentration of 0.18 μM and an exposure time of 24 h. Polarization-optical differential interference contrast and triple fluorescent staining (propidium iodide, Hoechst 33342 and acridine orange) revealed a decrease in cell density, metaphase arrest, and the occurrence of apoptotic bodies in the ESE-16-treated cells when compared to relevant controls. Treated cells also showed an increase in the presence of acidic vacuoles and lysosomes, suggesting the occurrence ofen© 2013 by the University of Wrocław, Poland. The original publication is available at : http://link.springer.comjournal/11658AntiproliferativeESE-16Esophageal carcinomaIn vitroApoptosisAutophagy2-ethyl-3-O-sulfamoyl-estra-1,3,5(10)16-tetraenePostprint Article