Sahle, M.Burgess, G.W.Boomker, Jacob Diederik Frederik2012-02-282012-02-2820122002Sahle, M & Burgess, GW 2002, 'Development of solid phase antigen for indirect ELISA for the detection of specific antibody responses to infection with Newcastle disease virus’. Onderstepoort Journal of Veterinary Research, vol. 69, no. 3, pp. 237-242.0030-2465http://hdl.handle.net/2263/18259The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.A simple and inexpensive method of antigen preparation by ultrafiltration was investigated using the V4 strain of Newcastle disease virus. The antigen designated XM300 was used in an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Newcastle disease virus in chicken serum. The assay was evaluated using both experimental and field sera, as well as reference control reactor and non-reactor sera. Antigen prepared by the ultrafiltration method was compared with antigen prepared by ultracentrifugation and the ultrafiltration antigen was found to react specifically with Newcastle disease virus antiserum in this ELISA system. This antigen preparation technique is also suitable for use in developing countries. The ELISA provides an excellent method for measuring antibodies in the early stages of infection in serum samples of experimentally infected chickens. More than 14.58% of the total serum samples which failed to be recognized as reactors by the conventional haemagglutination inhibition test were detected in the ELISA.© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineAntigen preparationIndirect ELISANewcastle disease virus (NDV)Veterinary medicine -- South AfricaAntigens -- South AfricaEnzyme-linked immunosorbent assayArticle