Buthelezi, Z.M.Pierneef, Rian EwaldBezuidt, Keoagile Ignatius OliverMakhalanyane, T.P.2026-04-162026-04-162025-07Buthelezi,, Z.M., Pierneef, R.E., Bezuidt, O.K.I. et al. 2025, 'High-quality metagenomic-assembled genomes from sea ice and seawater of the Southern Ocean', Microbiology Resource Announcements, vol. 14, no. 7, pp. 1-4. DOI: 10.1128/mra.01298-24.2576-098X (online)10.1128/mra.01298-24http://hdl.handle.net/2263/109597DATA AVAILABILITY : Whole genome shotgun data were deposited at DDBJ/ENA/GenBank under the BioProject accession PRJNA1167212 for assemblies and PRJNA1192238 for SRA submission. The BioSample accessions for the raw reads and genomes are listed in Table 1. EggNOG annotations for the MAGs are accessible through figshare: https://doi.org/10.6084/m9.figshare.28430819 28430819.We provide high-quality metagenome-assembled genomes (MAGs) derived from seawater and sea ice samples collected in the Southern Ocean. Several MAGs encode genes associated with dimethylsulfoniopropionate (DMSP) lyase activity and methane oxidation. This resource provides insights regarding the role of microbial communities in the production of key volatile compounds.en© 2025 Buthelezi et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.Southern OceanSeawaterSea iceDimethylsulfoniopropionate (DMSP)Methane monooxygenaseMetagenome-assembled genome (MAG)Article