Sahle, M.Burgess, W.G.Kidanemariam, AwokeBoomker, Jacob Diederik Frederik2012-03-062012-03-0620122002Sahle, M, Burgess, WG & Kidanemariam, A 2002, 'Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells’. Onderstepoort Journal of Veterinary Research, vol. 69, no. 3, pp. 201-2060030-2465http://hdl.handle.net/2263/18398The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran were incorporated in Dulbecco's modified Eagle's medium to encourage secondary viral multiplication without adversely affecting healthy Madin Derby bovine kidney cell growth.© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).Veterinary medicineCell cultureMDBK cellNewcastle disease virus (NDV)V4 strain replicationVeterinary medicine -- South AfricaVeterinary virologyNewcastle disease virus -- South AfricaArticle