Mbuayama, Kabuzi R.Taute, HelenaStromstedt, Adam A.Bester, Megan JeanGaspar, Anabella Regina Marques2022-07-252022-05Mbuayama, K.R., Taute, H., Stromstedt, A.A., Bester, M.J. & Gaspar, A.R.M. Antifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensin. Journal of Peptide Science 2022;28(5):e3383.doi:10.1002/psc.3383.1075-2617 (print)1099-1387 (online)10.1002/psc.3383https://repository.up.ac.za/handle/2263/86414Supporting Information: Table S1. Physicochemical properties and antifungal activity of OsDef2 derived peptides. Figure S1. Effect of sodium azide on the intracellular translocation of 5-FAM penetratin. C. albicans cells were pre-incubated with 5mM NaN3 for 30 min and treated with 5-FAM penetratin. The cells were counterstained with DAPI (blue) and visualized by confocal microscopy. Scale bars are 10 μm. Figure S2. DAPI stained images of 5-FAM-peptides in C. albicans. Cells were exposed for 50 min to 2.5 μM of 5-FAM-peptides, counterstained with DAPI (blue) and visualized by confocal microscopy. Top and bottom panel show same images at lower and higher magnification, respectively. Short arrow: fragmented nuclear DNA; long arrow: tubular-shaped DNA, in contrast to single and round nucleus observed in untreated cells. Images are representatives of three independent experiments, in duplicate. Scale bars are 10 μm.Please read abstract in the article.en© 2021 European Peptide Society and John Wiley & Sons, Ltd. This is the pre-peer reviewed version of the following article : Antifungal activity and mode of action of synthetic peptides derived from the tick OsDef2 defensin. Journal of Peptide Science 2022;28(5):e3383.doi:10.1002/psc.3383, which has been published in final form at : http://wileyonlinelibrary.com/journal/psc.Antimicrobial peptidesDefensinsMembrane permeabilizationReactive oxygen species (ROS)TicksPostprint Article