Uzelac, MirelaSladonja, BarbaraSola, IvanaDudas, SlavicaBilic, JosipaFamuyide, Ibukun MichaelMcGaw, Lyndy JoyEloff, Jacobus NicolaasMikulic-Petkovsek, MajaPoljuha, Danijela2024-09-132024-09-132023-07-21Uzelac, M.; Sladonja, B.; Šola, I.; Dudaš, S.; Bili´c, J.; Famuyide, I.M.; McGaw, L.J.; Eloff, J.N.; Mikulic-Petkovsek, M.; Poljuha, D. Invasive Alien Species as a Potential Source of Phytopharmaceuticals: Phenolic Composition and Antimicrobial and Cytotoxic Activity of Robinia pseudoacacia L. Leaf and Flower Extracts. Plants 2023, 12, 2715. https://DOI.org/10.3390/plants12142715.2223-7747 (online)10.3390/plants12142715http://hdl.handle.net/2263/98213DATA AVAILABILITY STATEMENT : Data are presented in the manuscript.SUPPLEMENTARY MATERIAL : TABLE S1: Pearson’s correlation coefficients (two-tailed) between total phenolic (TP), total non-flavonoids (TNF) and total flavonoids (TF) contents and antioxidant capacity (obtained by DPPH, ABTS, and FRAP assays).Black locust (Robinia pseudoacacia L.), an invasive tree in Europe, commonly known for its negative impact on biodiversity, is a rich source of phenolic compounds recognized in traditional medicine. Since the metabolite profile depends on the environment and climate, this study aimed to provide the first LC-MS phytochemical screening of the black locust from the Istria region (Croatia). The compounds were extracted from leaves and flowers with 70% ethanol and 80% methanol. Total phenolics (TP) and flavonoids (TF), as well as antioxidant capacity (AC) measured by ABTS (17.49–146.41 mg TE/g DW), DPPH (24.67–118.49 mg TE/g DW), and FRAP (7.38–77.53 mg TE/g DW) assays, were higher in leaf than in flower extracts. Higher TP and total non-flavonoid (TNF) values were displayed in ethanolic than in methanolic extracts. In total, 64 compounds were identified, of which flavonols (20) and hydroxycinnamic acid derivatives (15) were the most represented. Flavanols such as catechin dominated in leaf extracts, followed by flavonols, with kaempferol glucuronyl rhamnosyl hexosides as the main compound, respectively. Flower extracts had the highest share of flavones, followed by ellagitannins, with luteolin dirhamnosyl hexosides and vescalagin, respectively, being predominant. The extracts had good quorum sensing, biofilm formation prevention, and eradicating capacity. The results provided new insights into the phytochemical properties of R. pseudoacacia as the first step toward its potential pharmaceutical use.en© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.AntioxidantAntibacterialAntibiofilmAntifungalAnti-quorum sensingBlack locustPhenolicsSDG-03: Good health and well-beingSDG-15: Life on landArticle