Huchzermeyer, Karl David AugustColly, P.A.2015-11-022015-07K. D. A. Huchzermeyer & P. A. Colly (2015) Production of Koi Herpesvirus-Free Fish: Implementing Biosecurity Practices on a Working Koi Farm in South Africa, Journal of Applied Aquaculture, 27:3, 318-329, DOI :10.1080/10454438.2014.914997.1045-4438 (print)1545-0805 (online)10.1080/10454438.2014.914997http://hdl.handle.net/2263/50295Principles of biosecurity and disease surveillance long applied in the salmonid industries to produce certified disease-free stock can equally be applied to the production of koi herpesvirus-free koi, despite limitations in diagnostic ability to detect asymptomatic carrier fish. To ensure freedom from koi herpesvirus, there is a strong case for eradication of known infected broodstock. Maintaining a closed population of fish on a protected water source, adherence to strict biosecurity measures, and strategic sampling at a statistically relevant confidence level over a 2- year period can provide the guarantees needed for sale of certified disease-free fish. Propagation of such fish offers new entrepreneurial opportunities and has resulted in at least one breeder in South Africa achieving export certification for markets in the European Union.en© Taylor & Francis Group, LLC. This is an electronic version of an article published in Journal of Applied Aquaculture, vol. 27, no. 3, pp. 318-329, 2015. doi : 10.1080/10454438.2014.914997. Journal of Applied Aquaculture is available online at : http://www.tandfonline.com/loi/wjaa20.BreedingCarpCertificationCyprinus carpioDisease-freeKoi herpesvirus (KHV)Postprint Article