De Spiegeleer, BartVerbeke, FrederickD’Hondt, MatthiasHendrix, AnVan de Wiele, ChristopheBurvenich, ChristianPeremans, KathelijneDe Wever, OlivierBracke, MarcWynendaele, Evelien2016-09-192016-09-192015-03-17De Spiegeleer B, Verbeke F, D’Hondt M, Hendrix A, Van De Wiele C, Burvenich C, et al. (2015) The Quorum Sensing Peptides PhrG, CSP and EDF Promote Angiogenesis and Invasion of Breast Cancer Cells In Vitro. PLoS ONE 10(3): e0119471. DOI: 10.1371/journal.pone.0119471.1932-620310.1371/journal.pone.0119471http://hdl.handle.net/2263/56747S1 Table. List of quorum sensing peptides, repeatedly (n = 3) investigated for their effect on tumour cell invasion (Collagen Type 1 invasion assay).The role of the human microbiome on cancer progression remains unclear. Therefore, in this study, we investigated the influence of some quorum sensing peptides, produced by diverse commensal or pathogenic bacteria, on breast cancer cell invasion and thus cancer outcome. Based on microscopy, transcriptome and Chick Chorioallantoic Membrane (CAM) analyses, four peptides (PhrG from B. subtilis, CSP from S. mitis and EDF from E. coli, together with its tripeptide analogue) were found to promote tumour cell invasion and angiogenesis, thereby potentially influencing tumour metastasis. Our results offer not only new insights on the possible role of the microbiome, but also further opportunities in cancer prevention and therapy by competing with these endogenous molecules and/or by modifying people’s life style.en© 2015 De Spiegeleer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.Human microbiomeTherapyPreventionBreast cancerArticle