Farias Bezerra, Helen KalineDutra, Mateus JoseRoza, Ana Luiza Oliveira CorreaDe Freitas, Stephanie VargasKaminagakura, EstelaAnbinder, Ana LiaVazquez-Garduno, MarcelaRojas-Alcayaga, GonzaloEspinoza-Santander, Iris LuciaPinto, Ana Veronica OrtegaFarias, Danielle MachadoCarvalho, Elaine Judite de AmorimDe Castro, Jurema Freire LisboaLopes, Marcio AjudarteSantos-Silva, Alan RogerRobinson, LiamAbrahao, Aline CorreaRomanach, Mario JoseVan Heerden, Willem Francois PetrusMosqueda-Taylor, AdalbertoVargas, Pablo AgustinPerez, Danyel Elias da Cruz2025-09-182025-07Farias Bezerra, H.K., Dutra, M.J., Roza, A.L.O.C. et al. 2025, 'Calcifying odontogenic cyst : a detailed international multicentric study', Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, vol. 140, no. 1, pp. 100-112, doi : 10.1016/j.oooo.2025.01.731.2212-4403 (print)2212-4411 (online)10.1016/j.oooo.2025.01.731http://hdl.handle.net/2263/104386OBJECTIVE : To elucidate the histopathological, immunohistochemical, and ultrastructural characteristics of a large series of calcifying odontogenic cysts (COCs). STUDY DESIGN : This was a multicenter cross-sectional retrospective study of seven oral pathology department laboratories from four countries. Clinical, demographic, and radiographic data of the patients were collected. Histopathological, immunohistochemical, and microstructural analyses were based on formalin-fixed, paraffin-embedded tissues. All variables were described, and associations were analyzed using Pearson's chi-square and Fisher's exact tests. RESULTS : A total of 146 cases of COC were analyzed, including 141 central and 5 peripheral cases. Male patients were most involved, with a mean age of 27.8 years. The main clinical presentation was an asymptomatic swelling in the anterior maxilla. Radiographically, most cases appeared as a single well-defined unilocular radiolucency. Forty cases of COC were associated with other odontogenic lesions, including odontoma, ameloblastic fibroma, and odontogenic keratocyst. Immunohistochemical study showed cytoplasmic positivity for CK AE1/AE3, CK14, CK19, and Bcl-2 protein. Nuclear expression of β-catenin was observed in most cases. CONCLUSIONS : COC mainly affects adult males between the second and third decades of life. Asymptomatic maxillary swellings with diverse radiographic and histopathological presentations were noted. Moreover, COC may present associations with other odontogenic lesions, particularly odontomas in younger patients.en© 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies. All rights are reserved, including those for text and data mining, AI training, and similar technologies. Notice : this is the author’s version of a work that was accepted for publication in Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology,, vol. 140, no. 1, pp. 100-112, doi : 10.1016/j.oooo.2025.01.731.Histopathological characteristicsImmunohistochemical characteristicsUltrastructural characteristicsCalcifying odontogenic cyst (COC)Clinical aspectsRadiographical aspectsMicrostructural aspectsPostprint Article