Beylefeld, AmandaWambulawaye, PamelaBwala, Dauda GarbaGouws, JohanLukhele, Obed MookiWandrag, D.B.R. (Daniel)Abolnik, Celia2019-10-152019-10-152018-11Beylefeld A, Wambulawaye P, Bwala DG, Gouws JJ, Lukhele OM, Wandrag DBR, Abolnik C. 2018. Evidence for multidrug resistance in nonpathogenic Mycoplasma species isolated from South African poultry. Appl Environ Microbiol 84:e01660-18. https://DOI.org/10.1128/AEM.01660-18.0099-2240 (print)1098-5336 (online)10.1128/AEM.01660-18http://hdl.handle.net/2263/71820One hundred seventy-eight mycoplasma strains isolated from South African poultry flocks between 2003 and 2015 were identified by full-genome sequencing and phylogenetic analysis of the 16S rRNA gene and were classified as follows: Mycoplasma gallisepticum (25%), M. gallinarum (25%), M. gallinaceum, (23%), M. pullorum (14%), M. synoviae (10%), and M. iners (3%), as well as one Acheoplasma laidlawii strain (1%). MIC testing was performed on the axenic samples, and numerous strains of each species were resistant to either chlortetracycline or tylosin or both, with variable sensitivity to enrofloxacin. The strains of all species tested remained sensitive to tiamulin, except for one M. gallinaceum sample that demonstrated intermediate sensitivity. The mutation of A to G at position 2059 (A2059G) in the 23S rRNA gene, which is associated with macrolide resistance, was found in the South African M. gallisepticum and M. synoviae strains, as well as a clear correlation between macrolide resistance in M. gallinarum and M. gallinaceum and mutations G354A and G748A in the L4 ribosomal protein and 23S rRNA gene, respectively. No correlation between resistance and point mutations in the genes studied could be found for M. pullorum. Only a few strains were resistant to enrofloxacin, apart from one M. synoviae strain with point mutation D420N, which has been associated with quinolone resistance, and no other known markers for quinolone resistance were found in this study. Proportionally more antimicrobial-resistant strains were detected in M. gallinaceum, M. gallinarum, and M. pullorum than in M. gallisepticum and M. synoviae. Of concern, three M. gallinaceum strains showed multidrug resistance to chlortetracycline, tylosin, and oxytetracycline.en© 2019 American Society for MicrobiologyMycoplasmaMICPoultry16S rRNAAntibiotic resistance23S rRNADNA gyraseDNA topoisomeraseArticle