Havenga, MinetteWingfield, Brenda D.Wingfield, Michael J.Dreyer, Leanne LauretteRoets, FrancoisAylward, Janneke2021-06-022020-12Havenga M, Wingfield BD, Wingfield MJ, Dreyer LL, Roets F, Aylward J. Diagnostic markers for Teratosphaeria destructans and closely related species. Forest Pathology. 2020;50:e12645. https://doi.org/10.1111/efp.12645.1437-4781 (print)1439-0329 (online)10.1111/efp.12645http://hdl.handle.net/2263/80200Supplementary material: Table S1. Teratosphaeria isolates and their sequences used for phylogenetic analyses.Table S2. Microsatellite markers and panel designs for Teratosphaeria species.Table S3. BLASTn results of microsatellites in the Teratosphaeria genomes.Teratosphaeria foliar pathogens cause leaf and shoot blight on Eucalyptus trees in many parts of the world. Among them, T. destructans is one of the most aggressive pathogens causing defoliation of young Eucalyptus trees in tropic regions. Identification of T. destructans to species level is currently not possible based solely on morphological characteristics or ITS sequence data. The aim of this study was to assess T. destructans microsatellites and a newly developed T. epicoccoides microsatellite as a diagnostic tool to differentiate among T. destructans and several closely related foliar pathogens. Based on the number of markers that amplified, the T. destructans microsatellites allowed for the differentiation of T. destructans, T. epicoccoides,T. eucalypti,T. nubilosa,T. pseudoeucalypti and T. viscidus. These microsatellites provide a rapid and cost-effective diagnostic tool that will enable the identification of a large number of isolates important in disease surveys and inoculation trials.en© 2020 Wiley-VCH GmbH. This is the pre-peer reviewed version of the following article : Diagnostic markers for Teratosphaeria destructans and closely related species. Forest Pathology. 2020;50:e12645. https://doi.org/10.1111/efp.12645. The definite version is available at : http://wileyonlinelibrary.com/journal/efp.EucalyptusFoliar pathogensPopulation geneticsTeratosphaeria destructansDiagnostic markersPostprint Article