Williamson, Catherine C.Oberem, P.T.Poerstamper, C.De Waal, D.T.Matthee, O.Brett, O.L.Bigalke, R.D.2014-10-072014-10-0720141988Williamson, CC, Oberem, PT, Poerstamper, C, De Waal, DT, Matthee, O, & Brett, OL 1988, 'An ELISA using an SDS extract of Brucella abortus strain 99 as antigen to detect B. abortus antibodies in cattle sera', Onderstepoort Journal of Veterinary Research, vol. 55, no. 1, pp. 1-3.0330-2465http://hdl.handle.net/2263/42270The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.An ELISA was developed using an SDS extract of Brucella abortus as antigen to detect antibodies in cattle sera. The antigen was stable at 4 °C for at least 4 years and although it gave optimal results at a 1/4 000 dilution it could detect reactors at a 1/32 000 dilution. Based on comparative CF tests on 430 sera from negative herds and 187 sera from positive herds a reading of 0,07 or less using a 492 nm filter could be considered a negative reaction. The ELISA could be a useful test to supplement the CF test but further evaluation is still required.en©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).Veterinary medicineVeterinary medicine -- South AfricaArticle